Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Gas chromatography-mass spectrometry analysis of principal lipid-soluble components of Pinellia ternate fermented with Bacillus subtilis, Aspergillus niger and Meyerozyma guillermondii

Lin Wang¹, Jiabin Sun¹, Meiying Yue², Jin Wang¹ , Qinwan Huang¹, Zelun Lan³

¹College of Pharmacy; ²College of Basic Medicine, Chengdu University of Traditional Chinese Medicine, No. 1166, Liutai Road, Wenjiang District, Chengdu 611137; ³Sichuan Neautus Traditional Chinese Medicine Co. Ltd, No. 8 South Herui Road, High-Tech Zone, Chengdu 611731, China.

For correspondence:- Jin Wang Email: wangjin0816@126.com

Accepted: 12 May 2019 Published: 30 June 2019

Citation: Wang L, Sun J, Yue M, Wang J, Huang Q, Lan Z. Gas chromatography-mass spectrometry analysis of principal lipid-soluble components of Pinellia ternate fermented with Bacillus subtilis, Aspergillus niger and Meyerozyma guillermondii. Trop J Pharm Res 2019; 18(6):1305-1313 doi: 10.4314/tjpr.v18i6.23

© 2019 The authors.
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Abstract

Purpose: To study the differences in lipid-soluble compounds from naturally-fermented Rhizoma Pinelliae fermentata (BXQ) samples, and fermentation products of BXQ using pure cultures of Bacillus subtilis, Aspergillus niger, and Meyerozyma guillermondii.

Methods: First, unfermented BXQ (CTFJ-Q), traditional, naturally-fermented BXQ (CTFJ-H), and fermentation products of BXQ using pure cultures of Bacillus subtilis (XJFJ), Aspergillus niger (MJFJ), and Meyerozyma guillermondii (JMJFJ) were obtained. Their lipid-soluble components were then analyzed using gas chromatography-mass spectrometry (GC-MS) technology and principal component analysis (PCA).

Results: GC-MS results showed that there were 26, 24, 27, 31 and 32 types of chemical components in CTFJ-Q, CTFJ-H, XJFJ, MJFJ and JMJFJ, respectively. Furthermore, PCA revealed that samples obtained using fermentation with pure cultures of the three microorganisms had unique chemical components.

Conclusion: These results suggest that the microorganisms used for fermentation greatly influence the lipid-soluble components of BXQ. This finding is considered beneficial for the optimization of BXQ fermentation process.

Keywords: BanXia Qu, Pure culture fermentation, Pinellia ternate, Principal component analysis, Lipid-soluble components